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RESEARCH OF THE SAFETY OF THE FAT COMPONENT OF CAPSULATED FORMS OF SPECIALIZED PRODUCTS

https://doi.org/10.37493/2307-910X.2022.1.10

Abstract

Polyunsaturated fatty acids (PUFA) are widely used in high performance sports and fitness. It is worth noting their ability to reduce muscle damage and the inflammatory response after strength loads, improve the function of external respiration and normalize the athlete's body composition. Nevertheless, the direct use of PUFAs in food and beverages is limited by their low solubility in water and high sensitivity to oxidation, and therefore various antioxidants are introduced into their composition, a striking representative of which is astaxanthin, which, according to various scientific data, demonstrates effective use as a nutritional support, providing the body with the necessary elements for its construction and renewal, supplying the necessary amount of energy and substances involved in the regulation of physiological processes, as well as weakening vascular remodeling, suppressing the proliferation of vascular smooth muscle cells, improving the function of mitochondria. However, there are practically no works devoted to the study of various safety forms of PUFAs during storage. Nevertheless, they can be a potential source of danger to the body, due to the fact that during the oxidation process, oxidation products are formed, such as glycidol ethers, epoxides, secondary oxidation products, insoluble in petroleum ether, which have teratogenic, mutagenic and carcinogenic effects. In this regard, the aim of our study is to compare the biological effectiveness and safety of encapsulated forms of PUFAs with and without an antioxidant. The work uses generally accepted methods that normalize the safety and quality offats, regulated by the All-Russian Research Institute of Fats. The following indicators were investigated: determination of the concentration of epoxides; determining the acid number by reaction with concentrated phosphoric acid; peroxide number by reaction with sodium thiosulfate; determination of the total content of oxidation products insoluble in petroleum ether. As a result of the study of the fat component of the test samples, the acid number is 5.8 mg KOH / g, which is twice the maximum allowable value. These are not stable components, they react with each other or with other oxidation products and their content varies. The amount of epoxides in the studied capsules is 48.4 mmol / kg, which is approximately twice the permissible level, which cannot but cause concern for scientists around the world. The number of epoxides is not standardized in any of the regulatory and technical documents, but scientific studies have proven that these products are toxic. The safety indicators of the fat component regulated by the technical regulations of the Customs Union do not fully reflect the safety requirements for the fat component of biologically active additives, since there are no standards for the most important safety indicators of fats - the content of secondary oxidation products.

About the Authors

E. Y. Wolf
Saratov State Agrarian University
Russian Federation


I. V. Simakova
Saratov State Agrarian University
Russian Federation


A. A. Wolf
Saratov State Agrarian University
Russian Federation


References

1. E. Volf, I. Simakova, Yu. Eliseev, R. Perkel, E. Malyshev and A. Zinin Quality and safety problems of sports nutrition products Agronomy Research 18(3), 2020, р. 1888-1896

2. Котова, И. Б. Психология вкусовых ощущений и восприятий [Электронный ресурс] / И. Б. Котова, О. С. Канаркевич // Гуманизация образования. - 2009. - № 1. - С. 26-33

3. Banasaz S, Morozova K, Ferrentino G, Scampicchio M. Encapsulation of Lipid-Soluble Bioactives by Nanoemulsions. Molecules. 2020 Aug 31;25(17):3966

4. Hashimoto H, Arai K, Hayashi S, Okamoto H, Takahashi J, Chikuda M. The effect of astaxanthin on vascular endothelial growth factor (VEGF) levels and peroxidation reactions in the aqueous humor. J Clin Biochem Nutr. 2016 Jul;59(1):10-5

5. Lamazhapova GP, Syngeeva EV, Kozlova TS, Zhamsaranova SD. [Development of liposomal form of polyunsaturated fatty acid concentrate: ways of using in production of functional foods]. Vopr Pitan. 2017;86(1):76-84

6. Hajime Otani, "Site-Specific Antioxidative Therapy for Prevention of Atherosclerosis and Cardiovascular Disease", Oxidative Medicine and Cellular Longevity, vol. 2013, Article ID 796891, 14 pages, 2013

7. Iwamoto, T., Hosoda, K., Hirano, R., Kurata, H., Matsumoto, A., Miki, W., Kamiyama, M., Itakura, H., Yamamoto, S., Kondo, K. Inhibition of low-density lipoprotein oxidation by astaxanthin // Journal of Atherosclerosis and Thrombosis. - 2000. - V. 7, № 4. - P. 216-222

8. Fassett, R. G., Healy, H., Driver, R., Robertson, I. K., Geraghty, D. P., Sharman, J. E., Coombes, J. S. Astaxanthin vs placebo on arterial stiffness, oxidative stress and inflammation in 161 renal transplant patients (Xanthin): a randomised controlled trial // BMC Nephrology. - 2008. -V. 9, № 1. - P. 1-17.

9. Park, J. S., Chyun, J. H., Kim, Y. K., Line, L. L., Chew, B. P. Astaxanthin decreased oxi-dative stress and inflammation and enhanced immune response in humans // Nutrition & metabolism. - 2010. - V. 7, № 18

10. Speranza L., Pesce M., Patruno A., Franceschelli S., de Lutiis M.A., Grilli A., Felaco M. Astaxanthin treatment reduced oxidative induced pro-inflammatory cytokines secretion in U937: SHP-1 as a novel biological Target // Marine Drugs. - 2012. - V. 10, № 4. - P. 890-899.

11. Tominaga, K., Hongo, N., Karato, M., Yamashita, E. Cosmetic benefits of astaxanthin on humans subjects // Acta Biochimica Polonica. - 2012. - V. 59, № 1. - P. 43-47

12. Goswami, G., Chaudhuri, S., Dutta, D. The present perspective of astaxanthin with reference to biosynthesis and pharmacological importance // World Journal of Microbiology and Biotechnology. - 2010. - V. 26, № 11. - P. 1925-1939.

13. Kindlund, P. J. Astaxanthin // Nutrafoods. 2011. 10(2-3), 49-53

14. Lee, D.-H., Lee, Y. J., Kwon, K. H. Neuroprotective effects of astaxanthin in oxygen" glucose deprivation in SH-SY5Y cells and global cerebral ischemia in rat // Journal of Clinical Biochemistry and Nutrition. - 2010. - V. 47, № 2. - P. 121-129.

15. Nakagawa K., Kiko T., Miyazawa T., Carpentero Burdeos G., Kimura F., Satoh A., Miyazawa T. Antioxidant effect of astaxanthin on phospholipid peroxidation in human erythrocytes // British Journal of Nutrition. - 2011. - V. 05, № 11. - P. 1563-1571.

16. Fassett, R. G., Healy, H., Driver, R., Robertson, I. K., Geraghty, D. P., Sharman, J. E., Coombes, J. S. Astaxanthin vs placebo on arterial stiffness, oxidative stress and inflammation in 161 renal transplant patients (Xanthin): a randomised controlled trial // BMC Nephrology. - 2008. - V. 9, № 1. - P. 1-17

17. Fassett, R. G., Coombes, J. S. Astaxanthin in cardiovascular health and disease // Molecules. - 2012. - V. 17, № 2. - P. 2030-2048

18. Hussein, G., Sankawa, U., Goto, H., Matsumoto, K., Watanabe, H. Astaxanthin, a carote-noid with potential in human health and nutrition // Journal of Natural Products. - 2006. - V. 69, № 3. - P. 443-449

19. Preuss, H. G., Echard, B., Yamashita, E., Perricone, N. V. High dose astaxanthin lowers blood pressure and increases insulin sensitivity in rats: are these effects interdependent // International Journal of Medical Sciences. - 2011. - V. 8, № 2. - P. 126-138.

20. Yanai, H., Ito, K., Yoshida, H., Tada, N. Antihypertensive effects of astaxanthin // Integrated Blood Pressure Control. - 2008. - V. 1. - P. 1-3

21. Angwafor III, F., Anderson, M. L. An open label, dose response study to determine the effect of a dietary supplement on dihydrotestosterone, testosterone and estradiol levels in healthy males // Journal of the International Society of Sports Nutrition. - 2008. - V. 12, № 5. - P. 1-7.

22. Kanazashi, M., Okumura, Y., Al-Nassan, S., Murakami, S., Kondo, H., Nagatomo, F., Fu-jita, N., Ishihara, A., Roy, R. R., Fujino, H. Protective effects of astaxanthin on capillary regression in atrophied soleus muscle of rats // Acta Physiologica. - 2013. - V. 207, № 2. - P. 405- 415

23. Comhaire, F. H., Garem, Y. E., Mahmoud, A. H. M. E. D., Eertmans, F., Schoonjans, F. R. A. N. K.Combined conventional/antioxidant "Astaxanthin" treatment for male infertility: a double blind, randomized trial // Asian Journal of Andrology. - 2005. - V. 7, № 3. - P. 257-262.

24. Lombardo, F., Sansone, A., Romanelli, F., Paoli, D., Gandini, L., Lenzi, A. The role of an-tioxidant therapy in the treatment of male infertility: an overview // Asian Journal of Andrology. -2011. - V. 13, № 5. - P. 690-697

25. Bechelli, J., Coppage, M., Rosell, K., Liesveld, J. Cytotoxicity of algae extracts on normal and malignant cells // Leukemia Research and Treatment. - 2011. - V. 2011.

26. A. F., Andrei, S., Cernea, C., Taulescu, M., Catoi, C. Effects of astaxanthin supplementation on chemically induced tumorigenesis in Wistar rats // Acta Veterinaria Scandinavica. - 2012. -V. 54. - P. 50-56.

27. Чеканов К.А. Функционирование фотосинтетического аппарата микроводоросли HAEMATOCOCCUS PLUVIALIS (HCLOROPHYCEAE) при переходе в состояние гематоцисты. Дисс. канд. биол. наук. - М., 2016. - 193 с.

28. https://docplayer.ru/147936590-Klinicheskie-issledovaniya-shokolada-krasoty-esthechoc-poluchennogo-po-tehnologii-astacelle.html

29. Hashimoto, H., Arai, K., Hayashi, S., Okamoto, H., Takahashi, J., & Chikuda, M. (2016). The effect of astaxanthin on vascular endothelial growth factor (VEGF) levels and peroxidation reactions in the aqueous humor. Journal of clinical biochemistry and nutrition, 59(1), 10-15. https://doi.org/10.3164/jcbn.15-137

30. Chen Y, Li S, Guo Y, Yu H, Bao Y, Xin X, Yang H, Ni X, Wu N, Jia D. Astaxanthin Attenuates Hypertensive Vascular Remodeling by Protecting Vascular Smooth Muscle Cells from Oxidative Stress-Induced Mitochondrial Dysfunction. Oxid Med Cell Longev. 2020 Apr 14;2020:4629189. doi: 10.1155/2020/4629189. PMID: 32351673; PMCID: PMC7178508.

31. Рекомендации МГНОТ по диагностике и интенсивной терапии синдрома диссеминированного внутрисосудистого свертывания крови при вирусном поражении легких. Под редакцией проф. Воробьева П.А. и проф. Елыкомова В.А. Проблемы стандартизации в здравоохранении. 2020; 5-6.

32. Leckey JJ, Ross ML, Quod M, Hawley JA, Burke LM. Ketone Diester Ingestion Impairs Time-Trial Performance in Professional Cyclists. Front Physiol. 2017 Oct 23;8:806.

33. Синицкая Е. Н., Кокоричева Л. В., Манык Ф. М. Роль эйкозаноидов в жизнедеятельности человека // Молодой ученый. - 2018. - №50. - С. 97-99.

34. Benito, P., Caballero, J., Moreno, J., Gutierrez-Alcantara, C.,Munoz, C., Rojo, G., Garcia, S., Soriguer, F. 2006. Effects of milk enriched with omega-3 fatty acid, oleic acid and folic acid in patients with metabolic syndrome. Clin. Nutr. 25, 581-587.

35. Apte, M., Wilson, J., Lugea, A., Pandol, S. 2013. A starring role for stellate cells in the pancreatic cancer microenvironment. Gastroenterology , 144, 1210-1219

36. Irwin R., Eustis S., Stefanski S., Haseman J. 1996, Carcinogenicity of glycidol in F344 rats and B6C3F1 mice. Appl. Toxicol., 16, 201-209

37. Appel, K., Abraham, K., Berger-Preiss, E., Hansen, T., Apel, E., Schuchardt, S., Vogt, C., Bakhiya, N., Creutzenberg, O., Lampen, A. 2013. Relative oral bioavailability of glycidol from glycidyl fatty acid esters in rats. Arch. Toxicol., 87, 1649-1659.

38. Aasa, J., Granath, F., Tornqvist, M. 2019. Cancer risk estimation of glycidol based on rodent carcinogenicity studies, a multiplicative risk model and in vivo dosimetry. Food and Chemical Toxicology Vol.128, 54-60

39. Wang, W., Yang, J., Qi, W., Yang, H., Wang, C., Tan, B., Zhang, G. 2017. Lipidomic profiling of high-fat diet-induced obesity in mice: Importance of cytochrome P450-derived fatty acid epoxides. Obesity (Silver Spring) 25, 132-140

40. Li, P., Oh, D., Bandyopadhyay, G. , Lagakos, W., Talukdar. S., Osborn. O., Johnson. A., Chung. H., Mari.s M., Ofrecio. J. 2015. LTB4 promotes insulin resistance in obese mice by acting on macrophages, hepatocytes and myocytes. Nat. Med., 21, 239-247

41. Рогозин И.П. Кинетика изменения показателей безопасности жизнедеятельности при приготовлении во фритюре полуфабрикатов из теста. Актуальная биотехнология. - 2018. -№3 (26).- 2018. - С. 466-470

42. Стопский В., Меламуд Н., Куличенко Г., Естрина Ф. Научно-производственное объединение "Маслозирпром". 19. СП 2.3.6.1079; Авторское свидетельство СССР № 1040914, МПК7Г 01 N 33/02, G 01N 31/02. Метод количественного определения эпоксидных групп в жире

43. Ржехина В., Сергеева А.Г. Определение общего содержания продуктов окисления, нерастворимых в нефтяном комплексе: руководство по методологическим исследованиям, технологическому контролю и учету продукции в масложировой промышленности, ВНИИЖ. - Ленинград. - 1967. - 1007 с.

44. Определение суммарного содержания продуктов окисления, нерастворимых в петролейном эфире: руководство по методам исследования, технохимическому контролю и учету производства в масложировой промышленности / под общ. ред. В.П. Ржехина и А.Г. Сергеева. - Л.: ВНИИЖ, 1967. - Т. 1, кн. 2. - С. 1007.

45. Жиры и масла. Производство, состав и свойства, применение / Р. О'Брайен: пер.с англ. 2-го изд. В.Д. Широкова, Д.А, Бабейкиной, Н.С. Селивановой, Н.В. Маглы, - СПб.: Профессия, 2007. - 752 с.

46. Онищенко Г. Г. Санитарно-эпидемиологические требования к организации общественного питания, производству и обороту пищевых продуктов и продовольственного сырья в них. С.П. 2.3.6.1079-01. Москва, ФГУП "ИнтерСЕН". - 2001. - 22 с.

47. Ronald, J. Maughan, Quality Assurance Issues in the Use of Dietary Supplements, with Special Reference to Protein Supplements, Nutrition, Vol. 143 (11), 1843-1847.

48. Кашапов Р.И., Кашапов Р.Р. Особенности питания спортсменов-стайеров в циклических видах спорта // Вопр. питания. 2019. Т. 88, № 6. С. 12-21

49. Shahidi, F. 2005. Lipid oxidation: Theoretical aspects Bailey's industrial oil and fat products, Vol. 1, John Wiley & Sons, Inc., Hoboken, 3616 pp

50. Dong, J., Feng, X., Zhang, J., Zhang, Y., Xia, F., Liu, L., Jin, Z., Lu, C., Xia, Y., Papadimos, T., Xu, X. 2019. co-3 fish oil fat emulsion preconditioning mitigates myocardial oxidative damage in rats through aldehydes stress, Biomedicine & Pharmacotherapy, Vol.118 https://doi.org/10.1016/j.biopha.2019.109198.

51. Чеснокова Н. П., Понукалина Е. В., Бизенкова М. Н. Молекулярно клеточные механизмы инактивации свободных радикалов в биологических системах. Усп. соврем. Естествознания. - 2006. - 7: 29-36

52. Spagnuolo, M., Mollica, M., Maresca, B., Cavaliere, G., Cefaliello, C., Trinchese, G., Scudiero, R., Crispino, M. 2015. High fat diet and inflammation-modulation of haptoglobin level in rat brain. Front Cell Neurosci 9

53. Greene, J., Newman, J., Williamson, K., Hammock, B. 2000. Toxicity of epoxy acids and related compounds to cells expressing human soluble epoxide hydrolase/ Chem. Res. Toxicol., Vol. 13 (4). 217-226.

54. Goicoechea, E., Guillen, M. 2010. Analysis of hydroperoxydes, aldehydes and epoxydes by 1H nuclear magnetic resonance in sunflower oil oxidized at 70 and 100 оС Agric. Food Chem. Vol. 58. 6234-6245

55. Макаренко М., Малинкин А., Бессонов В., Саркисян В., Кочеткова А. 2018. Вторичные продукты окисления липидов. Оценка рисков для здоровья человека (статья 1). Проблемы питания. - 87 (6). - С.125-38

56. Гуляева. Л., Вавилин В., Ляхович В. Ферменты биотрансформации ксенобиотиков в химическом канцерогенезе, Новосибирск, / ГПНТБСО РАН, 2000. - С. 84

57. Симакова И., Перкель Р., Куткина М., Воловей А. Проблемы обеспечения безопасности жареных во фритюре продуктов быстрого приготовления. Вопросы питания. - 2015. Вып. 84 (5). - С.112-120

58. Симакова И., Воловей А., Куткина М., Перкель Р., под ред. Матисон, В. Оценка безопасности глубоких жиров в эксперименте на животных. Материалы III Международного форума «Инновационные технологии для обеспечения качества и безопасности пищевых продуктов. Проблемы и перспективы», V Международный научно-практический. Конференция "Безопасность и качество продуктов питания. Наука и образование". М.: Издательство-полигр. Москва, Центр МГУПП. - 2014. - С. 55-58

59. Goicoechea E., Guillen M.D. Analysis of hydroperoxides, aldehydes and epoxydes by 1H nuclear magnetic resonance in sunflower oil oxidized at 70 and 100 °C //j. Agric. Food Chem. 2010. Vol. 58. P. 6234-6245


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Wolf E.Y., Simakova I.V., Wolf A.A. RESEARCH OF THE SAFETY OF THE FAT COMPONENT OF CAPSULATED FORMS OF SPECIALIZED PRODUCTS. Modern Science and Innovations. 2022;(1):100-115. https://doi.org/10.37493/2307-910X.2022.1.10

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