The comparative analysis of traditional and modernized nutrient media for the cultivation of lactobacilli
https://doi.org/10.37493/2307-910X.2023.4.10
Abstract
Culture media for lactobacilli cultivation are used for their growth and reproduction in laboratory conditions, as well as in the production of dairy products. The composition of the developed nutrient medium includes: skimmed milk, yeast extract, lactose, lactulose, agar-agar, distilled water. This complex of components provides lactobacilli with essential nutrients, including carbohydrates, proteins, vitamins and minerals. The constituent components of the nutrient medium are affordable and cheap to purchase. The work involved the cultivation of various types of lactobacilli. (Lactobacillus acidophilus, Lactococcus lactis, Lactobacillus bulgaricus; Lactobacillus casei) on various nutrient media (MRS, Milk agar with prebiotics). It follows from the results of the experiments that the growth rate of lactobacilli on the nutrient medium "Milk agar with prebiotics" is higher in comparison with analogues of industrial production. The greatest intensity of probiotic growth is observed in yogurt starter culture, which includes probiotics of the following species: Lactococcus lactis; Lactobacillus bulgaricus; Lactobacillus casei, grown on the nutrient medium "Milk agar with probiotics", it is 3.7 * 109 CFU/ gr. The high economic efficiency of the developed nutrient medium in comparison with foreign analogues has been confirmedA formulation of a production nutrient medium necessary for the cultivation of probiotic microorganisms has been developed. It has been established that the growth rate of various lactobacilli on this medium is higher in comparison with analogues of industrial production. The high economic efficiency of the developed nutrient medium has been confirmed in comparison with foreign analogues.
About the Authors
E. I. Harina
North Caucasus Federal University
Russian Federation
Russian Federation
Elena I. Harina – Associate Professor of the Basic Microbiology Department, Faculty of Medicine and Biology
Stavropol, +79624134185
U. A. Malakhova
North Caucasus Federal University
Russian Federation
Russian Federation
Ulyana A. Malakhova – Laboratory Assistant at the Basic Microbiology Department, Faculty of Medicine and Biology
Stavropol, +79097507168
O. S. Butrimova
North Caucasus Federal University
Russian Federation
Russian Federation
Olesya S. Butrimova – Student at the Department of Applied Biotechnology, Faculty of Food Engineering and Biotechnology, specializing in "Industrial Biotechnology"
Stavropol, +79197303887
D. N. Chernov
North Caucasus Federal University
Russian Federation
Russian Federation
Denis N. Chernov – Student at the Department of Applied Biotechnology, Faculty of Food Engineering and Biotechnology, specializing in "Industrial Biotechnology"
Stavropol, +79614569531
References
1. Abilkhadirov AS, Abitaeva KG, Temirkhanov AZh, Dospaeva RT, Zakarya KD. Optimization of the nutrient medium for cultivating the lactic acid bacterium LACTOBACILLUS CASEI BM-4/17 B-RKM 0746 in a bioreactor. Bulletin of the Innovative Eurasian University. 2019;63-67. (In Russ.).
2. Begunova AV, Rozhkova IV, Zvereva EA, Glazunova OA, Fedorova TV. Lactic acid and propionic acid bacteria: Formation of products with bifidogenic and hypotensive properties. Applied biochemistry andmicrobiology. 2019;55(6):566-577. (In Russ.).
3. Borisenko EG, Rodriguez VI, Zuev RA, Molier A. Microbial nutriparapharmaceuticals based on complex plant raw materials. Young scientist. 2020. No. 22 (312). P. 423-424. (In Russ.).
4. Gaponova II, Shchetko VA, Romanova LB. Selection of a nutrient medium for cultivating and studying the growth dynamics of lactic acid bacteria Lactobacillus heiveticus. Microbial biotechnologies: Fundamental and applied aspects Collection of scientific papers. Minsk, 2021;13:42-51. (In Russ.).
5. Raskoshnaya TA, Semenikhina VF, Rozhkova IV, Begunova AV. Development of a nutrient medium and modes of Lactobacillus reuteri for obtaining a bacterial concentrate. Journal: Equipment and technology of food production. 2016;42(3). (In Russ.).
6. Timchenko LD, Penkova NI, Katunina LS. Comparative analysis of traditional nutrient media and a new cabbage medium for the cultivation of lactobacilli. Vestnik MGOU. Seriya: Natural Sciences. 2010;2:51-55. EDN:NCQXKP (In Russ.).
7. Omelchenko AV, Rzhevskaya V, Kryzhko AV, Panov DA. Effects of nanoselenium as a nutrient medium component on the main cultivation parameters and antagonistic activity of Lactobacillus strains. Proceedings of universities Applied chemistry and biotechnology. April 2021;11(1):125-135. https://doi.org/10.21285/2227-2925-2021-11-1-125-135
8. Belicova A, Mikulášová M, Dušinský R. Probiotic Potential and Safety Properties of Lactobacillus plantarum from Slovak Bryndza Cheese. J. BioMed. Res. Int. 2013:2013. https://doi.org/10.1155/2013/760298
9. Ayu BT, Chamnipa N, Apiraksakorn J. The Potential of an Inexpensive Plant-Based Medium for Halal and Vegetarian Starter Culture Preparation. Fermentation. 2023. https://doi.org/10.3390/fermentation9030216
10. De Man JC, Rogosa MA, Sharpe ME. A Medium for the Cultivation of Lactobacilli // Journal of Applied Microbiology. 2008. No. 23 (1). P. 130-135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.
11. L. Slattery [et al.] Invited review: Lactobacillus helveticus - a thermophilic dairy starter related to gut bacteria. J. of Dairy Science. 2010. Vol. 93. No. 10. P. 4435-4454.
12. Gujvinska SO, Paliy AP, Dunaeva OV, Paliy AP, Berezhna NV. Biotechnology production of medium for cultivation and lyophilization of lactic acid bacteria. Ukrainian Journal of Ecology. 2018;8(2):5-11. https://doi.org/10.15421/2018_30
13. Lysenko Yu., Machneva N., Smirnov A., Panin A., Koshchaev A. Biotechnology of Cultivation of Probiotic Lactobacilli. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies. 2020;12A4I:1-9. Available from: http://TUENGR.COM/V12/12A4I.pdf [Accessed 27 September 2023]. https://doi.org/10.14456/ITJEMAST.2021.72
Review
For citations:
Harina E.I., Malakhova U.A., Butrimova O.S., Chernov D.N. The comparative analysis of traditional and modernized nutrient media for the cultivation of lactobacilli. Modern Science and Innovations. 2023;(4):89-95. https://doi.org/10.37493/2307-910X.2023.4.10
Views:
342
Email this article 