msiСовременная наука и инновацииModern Science and Innovations2307-910XNorth-Caucasus Federal University10.37493/2307-910X.2024.1.8msi-1595Research ArticleТЕХНОЛОГИЯ ПРОДОВОЛЬСТВЕННЫХ ПРОДУКТОВTECHNOLOGY OF FOOD PRODUCTSХарактеристика физико-химических свойств, антиоксидантной, антимутагенной и репаративной активности нанокомплексов куркуминоидов с циклодекстринамиCharacteristics of physical and chemical properties, antioxidant, antimutagenic and reparative activity of nanocomplexes of curcuminoids with cyclodextrinsКапустинМ. А.KapustinM. A.

Максим Александрович Капустин – магистр биологических наук, старший научный сотрудник НИЛ прикладных проблем биологии

г. Минск; г. Ставрополь

+375297720526

Maksim A. Kapustin – Master in Biological Sciences, Senior Researcher of the Laboratory of Applied Biology

Minsk; Stavropol

+375297720526

maximkapustin84@gmail.comЧубароваА. С.ChubarovaA. S.

Анна Сергеевна Чубарова – кандидат биологических наук, доцент, старший научныйсотрудник НИЛ прикладных проблем биологии

г. Минск

+375295576722

Anna S. Chubarova – PhD in Biological Sciences, Associate Professor, Senior Researcher of the Laboratory of Applied Biology

Minsk

+375295576722

chubarova.hanna@gmail.comРжепаковскийИ. В.RzhepakovskyI. V.

Игорь Владимирович Ржепаковский – кандидат биологических наук, доцент, ведущий научный сотрудник межкафедральной научно-образовательной лаборатории экспериментальной иммуноморфологии, иммунопатологии и иммунобиотехнологии

г. Ставрополь

+79054164981

Igor V. Rzhepakovsky – PhD in Biological Sciences, Associate Professor, Leading Researcher of the Interdepartmental Scientific and Educational Laboratory of Experimental Immunomorphology, Immunopathology and Immunobiotechnology

Stavropol

+79054164981

78igorr@mail.ruПисковС. И.PiskovS. I.

Сергей Иванович Писков – кандидат биологических наук, ведущий научный сотрудникмежкафедральной научно-образовательной лаборатории экспериментальной иммуноморфологии, иммунопатологии и иммунобиотехнологии

г. Ставрополь

+78652956800

Sergey I. Piskov – PhD in Biological Sciences, Leading Researcher of the Interdepartmental Scientific and Educational Laboratory of Experimental Immunomorphology, Immunopathology and Immunobiotechnology

Stavropol

+78652956800

spiskov@ncfu.ruЕвдокимовИ. А.EvdokimovI. A.

Иван Алексеевич Евдокимов – доктор технических наук, профессор, заведующий базовой кафедрой технологии молока и молочных продуктов

г. Ставрополь

+79624030847

Ivan A. Evdokimov – Dr. Sci. (Techn.), Professor, Head of the Department of Milk and Dairy Products Technology

Stavropol

+79624030847

ievdokimov@ncfu.ruЛодыгинА. Д.LodyginA. D.

Алексей Дмитриевич Лодыгин – доктор технических наук, профессор, заведующий кафедрой прикладной биотехнологии

г. Ставрополь

+7652330318

Aleksei D. Lodygin – Dr. Sci. (Techn.), Professor, Head of the Department of Applied Biotechnology

Stavropol

+7652330318

allodygin@yandex.ruЦыганковВ. Г.CigankovV. G.

Василий Георгиевич Цыганков – кандидат медицинских наук, доцент, ведущий научныйсотрудник лаборатории комплексных проблем гигиены пищевых продуктов

г. Минск

+375293733551

Vasilii G. Cigankov – PhD in Medical Sciences, Associate Professor, Leading Researcher of Laboratory of Complex Problems of Food Hygiene

Minsk

+375293733551

vgz@tut.byДудчикН. В.DudchikN. V.

Наталья Владимировна Дудчик – доктор биологических наук, доцент, заведующийлабораторией микробиологии

г. Минск

+375172841370

Natalja V. Dudchik – Dr. Sci. (Biol.), Associate Professor, Head of Laboratory of Microbiology

Minsk

+375172841370

micro_sanitary@rspch.byАдамовичА. В.AdamovichA. V.

Анна Валерьевна Адамович – кандидат биологических наук, младший научный сотрудниклаборатории микробиологии

г. Минск

+375297662957

Аnna V. Adamovich – PhD, Junior Researcher, Laboratory of Microbiology

Minsk

+ 375297662957

sona_seg@mail.ruКурченкоВ. П.KurchenkoV. P.

Владимир Петрович Курченко – кандидат биологических наук, доцент, заведющий НИЛприкладных проблем биологии

г. Минск;  г. Ставрополь

+375296630347

Vladimir P. Kurchenko – PhD, Associate Professor, Head of the Laboratory of Applied Biology

Minsk; Stavropol

+375296630347

kurchenko@tut.byБелорусский государственный университет; Северо-Кавказский федеральный университетБеларусьBelorussian State University; North-Caucasus Federal UniversityBelarusБелорусский государственный университетБеларусьBelarusian State UniversityBelarusСеверо-Кавказский федеральный университетРоссияNorth-Caucasus Federal UniversityRussian FederationНаучно-практический центр гигиеныБеларусьScientific and Practical Center for HygieneBelarus202430052024018096Copyright © Капустин М.А., Чубарова А.С., Ржепаковский И.В., Писков С.И., Евдокимов И.А., Лодыгин А.Д., Цыганков В.Г., Дудчик Н.В., Адамович А.В., Курченко В.П., 20242024Капустин М.А., Чубарова А.С., Ржепаковский И.В., Писков С.И., Евдокимов И.А., Лодыгин А.Д., Цыганков В.Г., Дудчик Н.В., Адамович А.В., Курченко В.П.Kapustin M.A., Chubarova A.S., Rzhepakovsky I.V., Piskov S.I., Evdokimov I.A., Lodygin A.D., Cigankov V.G., Dudchik N.V., Adamovich A.V., Kurchenko V.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://msi.elpub.ru/jour/article/view/1595

Методом ИК-спектроскопии и термического анализа проведена характеристика спектральных свойств и термостабильности нанокомплексов куркуминоидов с циклодекстринами, рассчитаны изменения энергии активации термодеструкции куркуминоидов при формировании нанокомплексов. В модельной системе восстановления радикал-катиона АВТS˙+ определены значения IC50 для нанокомплексов куркуминоидов с β-циклодекстрином (КД:β-ЦД) и 2-гидроксипропил-бетациклодекстрином (КД:ГП-β-ЦД), полученных методом соиспарения и лиофилизации при соотношении 1:2, которые составили 1,076 г/л и 0,161 г/л соответственно. С использованием теста Эймса показано наличие антимутагенной активности препаратов КД:β-ЦД и КД:ГП-β-ЦД. При внесении в среду инкубации препарата нанокомплексов КД:ГП-β-ЦД 1:2 наблюдалось достоверное снижение частоты мутагенеза на 50% по сравнению с контролем. Применение нанокомплексов КД:β-ЦД 1:2 в качестве ранозаживляющего средства на модели лоскутной раны кожи у крыс линии Вистар показало течение регенерацтивного процесса по пути органотипической регенерации.

The spectral properties and thermal stability of cyclodextrins:curcuminoids nanocomplexes were characterized by the use of the methods of IR spectroscopy and thermal analysis. The changes in the activation energy of thermal destruction of curcuminoids during the formation of nanocomplexes were calculated. In the model system for the reduction of the radical cation ABTS˙+, IC50 values were determined for nanocomplexes of curcuminoids with β-cyclodextrin (Curc:β-CD) and 2-hydroxypropyl-beta-cyclodextrin (Curc:HP-β-CD), obtained by co-evaporation and lyophilization at a ratio of 1:2, which were 1.076 g/L and 0.161 g/L, respectively. Using the Ames test, the presence of antimutagenic activity of the drugs Curc:β-CD and Curc:HP-β-CD was demonstrated. When Curc:HP-β-CD 1:2 nanocomplexes were added to the incubation medium of the drug, a significant decrease in the frequency of mutagenesis was observed by 50% compared to the control. The use of Curc:β-CD 1:2 nanocomplexes as a wound-healing agent on a skin patch wound model in Wistar rats showed the course of the regenerative process along the path of organotypic regeneration.

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The authors declare that there are no conflicts of interest present.